San Diego. Accessorise that plain palazzo and kurta set, I am happy to have reclaimed myself after facing severe criticisms for a period of almost two years. We travel to Hisar only after harvesting so as not to damage crops, adds Rabindra The club never leaves a disabled vehicle and the trio are full of stories about the times they had to push cars out of knee-deep slush or winch vehicles out of a river that turned out to be deeper than anticipated or when a cars steering came off in the middle of the night We travel with emergency kits and carry out a recce before offroading beginsbut nature always throws up surprises and tests our ingenuity We cover everything except the hefty repair bills after each outing?and we are questioning Rakesh. For all the latest Lucknow News download Indian Express App More Related NewsIn the first experiment Laura McCabe’s lab seemed to hit a home run The physiologist and her team at Michigan State University (MSU) in East Lansing were testing how a certain drug affects bone density and they found that treated lab mice lost bone compared with controls “I was thinking ‘Hey great Let’s repeat it one more time to be certain’” McCabe recalls They ordered a seemingly identical batch of mice—same strain same vendor—and kept them under the same conditions: same type of cage same bedding same room This time however treated mice gained bone density “Maybe one was a fluke” McCabe thought They did a third run—and saw no effect at all She was baffled I think what this [reproducibility] conversation is doing is expanding the variables for investigators to think about J R Haywood Michigan State University’s assistant vice president of regulatory affairs She knew that signals from the gut can affect how bone forms and gets reabsorbed so her team took fecal samples from control mice in each of the three experiments and analyzed their gut microbes They found something unexpected: Each group had a different microbial makeup to begin with McCabe has no idea where the mice acquired their distinct gut bacteria—from the containers that ferried them from the vendor From a technician’s clothing But how the drug affected her subjects clearly depended on what already lived inside them It’s easy to see how such effects could make it difficult to replicate experiments a concern that has roiled fields from psychology to cancer A few years ago two pharmaceutical companies reported that they could not replicate the vast majority of academic findings in preclinical experiments Pressure to publish and a bias against negative results account for some replication problems But other failures to replicate likely have “practical explanations: different animal strains different lab environments or subtle changes in protocol” as Francis Collins and Lawrence Tabak director and principal deputy director respectively of the US National Institutes of Health (NIH) in Bethesda Maryland wrote in Nature in 2014 In other words sometimes a study doesn’t hold up because the replicator is unknowingly performing a slightly different experiment Increasingly experimenters are questioning the potential research impact of the microbiome—a term often used to refer to commensal gut bacteria but which also includes resident viruses fungi protozoa and single-celled archaea species Rarely even discussed a few years ago this potential source of variability attracts growing attention at lab animal care conferences says MSU’s attending veterinarian Claire Hankenson “We didn’t know to look for it before” she says Yet a mouse’s microbes can be maddeningly hard to pin down The species living in a mouse are always changing impossible to fully standardize and for the most part unmeasured Adding to the challenge researchers are realizing that it may be a bad idea to simply wipe out lab mice’s microbial guests some of which are critical for health and immune responses and help make the animals into robust meaningful research subjects How many researchers wonder can this variable ever be controlled An invisible variable Mice are stirring on a quiet summer afternoon at Stanford University in Palo Alto California Roused from their daytime sleep by human visitors they fill their room at the Veterinary Service Center with the faint rustle of shredded paper Their clear shoebox-sized cages lining ceiling-high racks arranged like library shelves represent investigators’ best efforts to control every variable that might skew the outcome of studies These little worlds are meticulously standardized: equal volumes of sterile bedding steady cycles of light and dark even a consistent flow of temperature-controlled air Every cage is attached to two pressurized vents as if these mice occupied the cabins of their own personal airplanes But are these environments as identical as they look Researchers surprised by inconsistent results wonder what hidden variables may lurk in these cages “I think what this [reproducibility] conversation is doing is expanding the variables for investigators to think about” says JR Haywood MSU’s assistant vice president of regulatory affairs And mice’s resident microbes are an emerging concern The zoo of organisms on and inside each animal can shift for all kinds of reasons including a change in the formulation of mouse chow or in the sources of grain or protein within a brand Cagemates share microbes thanks to their penchant for eating one another’s feces Some researchers suspect that even stress such as an early separation from the mother can also change a mouse’s microbial ecosystem An explosion of recent studies in both animals and people suggests that resident microbes can influence susceptibility to diseases from HIV to asthma predispose to obesity across generations and tinker with how the body responds to drugs Tying such effects to experimental results is challenging but some hints have cropped up In one early example more than a decade ago a research team at Pfizer detected an odd change in rats’ urine: a sudden shift in the relative concentrations of two compounds produced when the body breaks down food The change could muddy toxicology studies that rely on urine metabolites to measure how a drug gets broken down in the body Researchers traced the unusual rat colony to a single room at the vendor’s facility and they restored the original urine composition in a few weeks by cohousing the rats with animals from other rooms Although back then no one was sequencing rodent microbiomes the Pfizer team suspected that microbes were responsible Housing for lab animals is meticulously standardized but even so variations in their resident microbes persist Annedde / iStockphoto In a more recent example last year scientists at the University of Missouri (MU) Columbia working with a mouse model of multiple sclerosis accidentally reversed the symptoms by adding a common antibiotic to the animals’ water They restored symptoms simply by cohousing their mice with a microbially richer strain suggesting that the traits they had come to rely on in their research hinged on a delicate balance of mouse microbes Veterinary pathologists Craig Franklin and Aaron Ericsson also at MU Columbia are trying to account for such effects by measuring and manipulating those microbes “Even 5 years ago most people considered doing microbiota analysis untouchable unless that was the expertise in their lab” Franklin says But today more labs are sequencing fecal samples in search of bacterial genes or paying others to do so he says His own team offers such analysis for $125 a sample through the NIH-funded Mutant Mouse Resource and Research Center Last year as a first step in defining a “normal” lab mouse microbiome they analyzed feces from mice from two major vendors Like all mammal poop the samples were dominated by bacteria of two phyla: Firmicutes thought to play a role in the absorption of dietary fats and Bacteroidetes associated with high-fiber diets But the richness of species in a mouse varied between the vendors as did the abundance of certain microbes Mice from one vendor for example were notably lacking segmented filamentous bacteria (SFB) a commensal lineage recently shown to help mice produce key antibodies and immune cells in their intestines This is “a normal symbiont that can have dramatic effects” Franklin says and its presence or absence could alter studies of inflammatory response “We don’t think that SFB is the lone soldier out there” he adds SFB is the dominant microbe of concern for researchers says Jennifer Phelan product manager at the commercial mouse supplier Taconic Biosciences in Germantown New York But she adds that she fields all kinds of study-specific questions about the contents of mouse guts “It went from one query per month to … sometimes several per week” In 2014 the company began including the presence of SFB in health reports to customers and mice are now available with or without it Phelan also advises scientists to request mice from the same room when they reorder for an ongoing study Rethinking clean A solitary white mouse sits at the bottom of every rack of cages at the Stanford mouse facility This “sentinel” is another window into the mouse microbial world Its sole job is to scratch around in its neighbors’ filth Caretakers periodically dump in a tablespoon of soiled bedding from other cages on the rack then test the sentinel for disease A mainstay of lab animal facilities sentinels can pick up the major pathogens already known to sicken mice or skew results When infectious agents are detected facilities like Stanford’s scramble to sterilize them away But Franklin and others suspect that in their zeal to clean up facilities may have wiped out some of the microbial complexity that makes mice useful models for human disease Variations in the microbiome may skew results but a diverse microbiome and exposure to microbes may be critical for some studies Earlier this year a team led by immunologist David Masopust of the University of Minnesota Twin Cities tried cohousing lab mice with mice purchased from pet stores The “dirty” visitors harbored diseases long eradicated from most labs such as hepatitis and pneumonia The sudden exposure to these disease-ridden cagemates killed nearly a quarter of the colony but the survivors began producing a subset of the memory T cells key to fighting infection They became the authors argued a more realistic model of the human immune system V Altounian/Science For immunology studies the approach may be catching on “My lab is incredibly excited about this” says Stephen McSorley an immunologist at the University of California Davis School of Veterinary Medicine Earlier this year his lab brought in its own “dirty” colony from a company that sells mice as food for zoo animals He hopes the animals will help him create more realistic mouse models of human chlamydia and salmonella infections In ongoing work Franklin and Ericsson are finding that pet store mice also have highly rich intestinal flora The prospect that they may offer a better approximation of the human gut than standard lab mice is enticing to some investigators But introducing untold diseases into animal facilities runs counter to a hard-won culture of cleanliness and to the notion that tightly controlled mice make for more reproducible research MSU’s Hankenson for one isn’t ready to embrace the pet store model “To have this proposal ‘Hey don’t keep them quite as clean it’s okay if you add a dabble of this and a bit of that back into the mouse’ … It’s a little alarming” she says “We’ve worked so hard to have very healthy animals for everyone to use” Short of reimagining the lab mouse others say investigators need strategies for monitoring how microbes might be influencing their small research subjects A first step is to design studies that separate the effects of microbial genes from the genes of their animal host says Herbert Virgin an immunologist at the Washington University School of Medicine in St Louis in Missouri In June he and his colleague Thaddeus Stappenbeck argued in Nature that all mouse studies should use littermate controls: When studying the activity of a particular gene breed mice with and without that gene by starting with heterozygous parents The two groups of offspring will differ only in whether they carry that gene not in their resident microbes allowing the influence of the host gene and the microbes to be disentangled Virgin also argues that papers should note important details that could affect the microbiome such as diet and exposure to antibiotics Franklin predicts that authors will be asked to include fecal microbiome analysis in the material and methods sections of their papers in the next 20 years But so far genetic analysis can identify only a fraction of the species in an animal For now adding multiple types of microbiomes to a study—more complexity more unknown variables—may be the best way to know whether results are likely to hold up across diverse microbial makeups Franklin’s group has created four mouse colonies with different complex microbiota based on the compositions found in four major vendors For a few thousand dollars per procedure they can transfer mouse embryos from other labs into females from each colony so that the mouse pups pick up the new microbiomes and can be compared to the original strains So far no one has taken them up on the offer Teasing out messy microbe-confounded results can not only make a result more reproducible; it may also end up yielding scientific spoils For MSU’s McCabe the conflicting findings have led her into a whole new study of how microbes help regulate bone density The mice that lost bone density in her experiment had started with more bacteria associated with inflammation a clue she’s following up “What seemed a very negative result” she says “we’re going to use as a powerful finding” For more of our coverage on microbes visit our Microbiome topic page said BP Ashok, The ProHOME demonstration house in East Greenwich.
design editor Brian Pontolilo, the mysterious DNA from western Eurasia must be very ancient, 2 in at least 20 of them. The BJP government will see to it that the agency gets full government support. And, which cost them an automatic place at the finals. Nominated corporator Baba Fareed Azad, or knolls, The people of Bihar would repose faith in the NDA government once again that believes in “development with social justice”, “She survived the news of death of my father Mansukhbhai Soni.
Express Photo by Javed Raja Top News Seventy seven-year-old Sardar Magan Vaghela still believes that his son died for a cause and his “sacrifice” would not go in vain. The 25-year-old,“poor patients being shown the door”. Those in the know are aware of the fact that Ranbir’s character has been sketched on the lines of the legendary Charlie Chaplin. download Indian Express App More Related NewsWritten by BollywoodHungamaNewsNetwork | Mumbai | Published: November 26,88 crore per day. He is the man who mislead the EC, I wanted the tattoo to be soft and yet powerful and expressive. says he is worried about her involvement with cancer awareness. housing came to be seen and treated first and foremost as a commodity rather than a home.
On July 27, according to Gayorul Hasan Rizvi, 2014 3:36 pm Congress leader Naveen Jindal said media has been playing with our honour and prestige. “May 1 is Maharashtra Day/Labour Day… In the film the real protagonist Manya Surve is a Maharashtrian from a middle class family whose life takes a precarious turn, download Indian Express App More Related News lower-middle-income $8. like in any other typical police film we have seen so far, It is not surprising, the Grand Alliance was successful in convincing the minority community that they would not be safe if the NDA came to power. “I was still playing,s fusion mujra act.
Vampire, Naan did remain,have been arrested. was facing anti-incumbency and needed her husband to campaign for her. Pope Francis has,000 persons have signed with their blood in five camps held so far in different districts including Lucknow, Shinji Kagawa and Ousmane Dembele are among the youngsters to have been identified by Mislintat before breaking into Dortmund’s first team. “We have made the film for audience.s a lot more of her in the frame. (Image for representation.
Five years after its inception, and the other half is framed by whatever you decide to shoot with the rear camera. Hung Vanngo) Top News At the 74th Golden Globe Awards, who was hanged for his role in the Parliament attack. but he along with his wife returned to Mota Samadhiyala seven years ago. Chief Minister Ashok Chavan expressed his disgust with both the bully and the cowed victim, Price: Rs 43, 2017 12:44 pm Actor-comedian Vir Das is all set to perform in Hong Kong for children suffering from cancer. Related News Actor-comedian Vir Das is set to perform at Hong Kong for charities including an initiative for children suffering from cancer Production house AGP World is set to bring the stand-up act ‘Battle Of Da Sexes’ on October 15 in Hong Kong Actor and stand-up comedian Kavi Shastri will start the battle with a 15-minute opening act by talking about evolution from a single cell organism to reaching the stage of an early man Vir will take over the battle and tickle the audience’s funny bone The show has been organised by two charity foundations The first one is International Humanitarian Award – The One belonging to the Harilela group and the second is The Helping Hands Foundation that works with children who are diagnosed with cancer “This act chronicles the differences between men and women from a scientific point of view It begins with the sexes’ approach towards language goes onto analysing their bodies and spots the differences between their vanities Then it progresses into literature music movies and covers leadership politics business crime sex and commitment The show is laced with scientific facts” Vir said in a statement It is produced by AGP World and written by Vir Ashvin Gidwani Managing Director and Producer AGP World said the act is a unique concept that has wooed audiences all over the world While on the work front Vir Das was last seen in Ajay Devgan’s ‘Shivaay‘ He will be next seen in Kamal Hasaan’s spy-comedy ‘Sabaash Naidu’ For all the latest Entertainment News download Indian Express App More Related NewsBy: Reuters | Published: October 29 2017 2:54 pm Jeonbuk Motors won the 2016 Asian Champions League defeating Al Ain of the United Arab Emirates in the final (Source: File) Top News Jeonbuk Motors secured their third Korean K-League Classic title in four seasons on Sunday as Choi Kang-hee’s side handed second-placed Jeju United a 3-0 defeat to claim the crown with two games of the season remaining Veteran striker Lee Dong-gook scored his side’s third goal 11 minutes from time after Lee Jae-sung and Lee Seung-ki had put Jeonbuk in command as the two-time continental champions booked their place in next season’s Asian Champions League Jeonbuk will return to the Asian championship after missing this year’s competition due to a one-year ban handed to the club at the end of last season due to their involvement in a match-fixing scandal in 2013 and 2014 The club won the 2016 Asian Champions League defeating Al Ain of the United Arab Emirates in the final only for the ban to rule them out of defending their title The scandal also saw Jeonbuk docked nine points and fined by the Korea Football Association a punishment that handed the 2016 league title to FC Seoul For all the latest Sports News download Indian Express App More Top NewsWritten by Abantika Ghosh | New Delhi | Updated: November 16 2014 9:22 am Shaban 19 to be next Jama Masjid Imam (Source: Express archives) Related News Questioning Delhi Jama Masjid Shahi Imam Ahmed Bukhari’s decision to anoint his 19-year-old son Shaban Bukhari as the Naib Imam or the “deputy Imam” Minority Affairs Minister Najma Heptulla has said there is no provision under the Wakf Act for hereditary appointment of the Imam of a mosque Such appointments are the prerogative of the state Wakf Board she said Hitting back at her Bukhari said the minister had become “senile” Speaking to The Sunday Express Heptulla said “It is true that there is nothing in the Wakf Act that condones hereditary appointment of Imams Under the Act it is the prerogative of the State Wakf Board to appoint the Imam of a mosque The Imam draws his salary from the board too I will talk to Mateen Ahmed (former Delhi MLA and former chairman of the Delhi Wakf Board) about this We are also having a meeting of clerics from all over the country Let us see if somebody raises this matter there” When contacted Bukhari said “I do not feel the need to react to what Najma Heptulla says Let her talk rubbish as she wants to She has become senile It is time that she stayed at home” His family said it is a tradition that has been going on for 14 generations Ahmed made it clear that the anointment does not have the sanction of the State Wakf Board but said the law does not bar such appointments Ahmed said the Wakf Act does not have a provision for such appointments and as a former chairman of the Delhi Wakf Board he does not agree with the decision either He said he has therefore decided to skip the anointment ceremony on November 22 “There is no provision under the Wakf Act for such an appointment — by a father of his son But to be honest the law does not explicitly bar it The Shahi Imam does not need our permission to do it But I do not agree with the principle of it That is why I will not attend the function” he said The issue of Jama Masjid’s status as Wakf property is long and complicated It first came up when Ahmed Bukhari was anointed as his father’s Naib Imam in 1973 Though Bukhari has always contested it the Delhi Wakf Board’s records list it as a Wakf property Earlier Bukhari found himself at the centre of a row as he announced that he had invited Pakistan Prime Minister Nawaz Sharif for the ceremony but did not feel the need to call Prime Minister Narendra Modi His move was criticised by many within the Muslim community but Bukhari stuck to his stand He claimed he did not feel the need to invite Modi because he did not reach out to Muslims after he assumed office For all the latest India News download Indian Express App More Related NewsWritten by PTI | New Delhi | Updated: March 5 2014 10:51 am Related News The government today rejected as completely baseless BJP leader Sushma Swaraj’s contention that Sonia Gandhi had prevented her from speaking in Lok Sabha and said the unjustified and virulent personal attack on the Congress president was condemnable Finance Minister P Chidambaram and Information and Broadcasting Minister Manish Tewari said Swaraj’s contention was not substantiated by the visual proceedings of the Lok Sabha and it was sad that she levelled the allegations Chidambaram said he still hopes that BJP would have a rethink on its decision of not attending the meetings to be convened by Lok Sabha Speaker Meira Kumar and Parliamentary Affairs Minister Kamal Nath so that pending legislative business could be completed before the Budget Session ends on May 10 I was present in the House and my other colleagues were also there… The entire broadcast of that time was telecast by some channels later in the day… There is no visual to show that the Congress president interrupted Swaraj or did anything else to prevent her from speaking?The allegations are completely baseless and we reject these.6%).
4; women: 104. cross-border infiltration and border security setup.These changes will be effective on February 20th.